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GAPDH Recombinant Rabbit mAb

Cat No.: RMA02916
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  • Western blot analysis of Anti-GAPDH antibody(RMA02916) Gel: 15%SDS-PAGE Lysate: 20 μg Primary antibody: RMA02916(GAPDH antibody)at dilution 1:1000 Secondary antibody: Goat anti rabbit IgG (SPA10002)at 1:1000 dilution

Product Name: GAPDH Recombinant Rabbit mAb
Cat No.: RMA02916
Clonality: Monoclonal
Species Reactivity: Human, Mouse, Rat, Chicken, Zebrafish, Monkey, Xenopus tropicalis
Tested Applications: WB,IHC,ICC/IF,FC
Recommended Dilution: WB: 1:2000
IHC: 1:200
ICC/IF: 1:100
FC: 1:50
IP: 1:20
Size: 30ul 50ul 100uL
Format: Liquid
Source: Rabbit
Purification Method: Affinity Purification
Isotype: IgG
Conjugate: Un-conjugated
Storage: Store at -20°C. Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide a
Immunogen: Recombinant protein of human GAPDH
Calculated Molecular Weight: 36 kDa
Observed Molecular Weight: 36 kDa
GenBank Accession Number: P16858
Gene ID (NCBI): 14433
Synonyms: G3PD; GAPD; HEL-S-162eP
Background: This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The encoded protein was originally identified as a key glycolytic enzyme that converts D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Subsequent studies have assigned a variety of additional functions to the protein including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Alternative splicing results in multiple transcript variants. Many pseudogenes similar to this locus are found throughout the mouse genome. [provided by RefSeq, Jan 2014]
Category: Primary Ab
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